Fig. 5.

Malt1 regulates LPA-induced NF-κB activation but not MAPK or Akt activation. (A and B) Malt1^+/+, Malt1^+/−, or Malt1^−/− MEFs were preincubated with cycloheximide and then stimulated with 10 μM LPA (A) or 10 ng/ml TNF-α (B) for the indicated times. Total extracts were prepared and subjected to immunoblotting with an antibody against IκB-α and reprobed with anti-β-actin antibody for protein loading control. (C and D) Malt1^+/+, Malt1^+/−, or Malt1^−/− MEFs were stimulated with LPA (C) or TNF-α (D) as described above; nuclear extracts were prepared and incubated with radiolabeled oligonucleotides against NF-κB or NF-Y as a loading control. (E) Malt1^+/− or Malt1^−/− MEFs were stimulated with 10 μM LPA as indicated, and cell extracts were immunoblotted with antibodies against phospho-p38, phospho-p44/p42 (phospho-Erk), phospho-Jnk, or phospho-Akt. After stripping, membranes were reprobed with antibodies against p44/p42 (Erk) as a loading control. Results are representative of four different experiments.