Fig. 2.

ZAP cosediments with the exosome components. 293TRex cells expressing various versions of ZAP were lysed in the hypotonic buffer. The lysates were fractionated by sucrose (A) or glycerol (B) velocity gradient centrifugation. Equal aliquots of each fraction were subjected to SDS/PAGE and were Western blotted with the 9E10 anti-myc antibody or antibodies against the exosome components hRrp40p, hRrp41p, or hRrp46p. Total RNA was extracted from equal aliquots of each fraction, and the 28S and 18S rRNAs were detected by ethidium bromide staining