Fig. 2.

In vitro differentiation of NGN3⁺ cells. (A) Schematic of the coculture strategy for assessing developmental potential of sorted fraction II single cells. (B–D) Expression of indicated β cell markers after coculture on PA6 feeders of fraction II cells from WT mice. (E and F) Coexpression of insulin and EGFP after coculture on PA6 feeders of fraction II cells from MIP-EGFP mice. (G) RT-PCR analysis of sorted cells before in vitro culture. (H) RT-PCR analysis of fraction II cells after coculture with MEFs for 2 days.