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FACS and in vitro culture of E15.5 ngn3–/– pancreatic cells. (A) FACS analysis of pancreatic cells from control (WT) and ngn3–/– embryos revealed reduced cell numbers in fractions II and III (circled) from ngn3–/– pancreas. (B) After 4 days of coculture, ngn3–/– fraction II cells expressed detectable E-cadherin (green), an epithelial marker, but failed to produce detectable C-peptide (red).
