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DCs, but not B cells, cluster surface MHC II and CD86. DC (A) were matured for 6–12 h, after which they or 24-h B blasts (B) were prepared for IF microscopy. Confocal images of a single plane are shown. (Scale bar, 5 μm.) Areas boxed in the right, merged panels are shown at higher magnification in individual colors in the left (MHC II, green) and middle (CD86, red) panels. Arrows show examples of PM regions with clustering of both molecules.
