Fig. 2.

DCs, but not B cells, recruit irrelevant MHC II to site of bead contact. After adhering I-E-GFP-expressing DCs (A) or B blasts (B) to coverslips, Ab-coated beads were added and incubated with cells at 37°C for 10 min, followed by fixation and fluorescence microscopy. (Upper) I-E beads. (Lower) I-A beads. (Scale bars, 5 μm.) Confocal images of a single plane are shown (Left), and DIC images show bead position (Right). Arrows show sites of bead contact and/or I-E enrichment.