Fig. 1.

Induction of clots in penetrating arterioles by photothrombosis. (A) Schematic of experimental set-up. Green laser light is focused into a penetrating arteriole by a microscope objective concurrent with TPLSM of the vasculature. (B and C) Maximal projections in the tangential (B) and sagittal (C) directions of image stacks through a penetrating vessel clot; schematics indicate the directions and volumes of projection. The clot is visualized as a dark mass in the target vessel that is often surrounded by bright areas of stalled, labeled plasma. The images are inverted for clarity. B Inset shows tangential view before irradiation.