Figure 1.

Comparison of β-chemokine production by CD8⁺ T cells from HIV-1-infected cases stratified by CD4⁺ T-cell count and uninfected cases. CD8⁺ T cells were isolated from HIV⁺ individuals with CD4⁺ T-cell counts less than (Group A) or greater than (Group B) 200 cells per μl. CD8⁺ T cells were also obtained from uninfected donors (Group C) for further comparisons. The cells were isolated from freshly collected PMBCs by immunomagnetic beads, and 2 × 10⁶ cells per ml were activated with PHA (1 μg/ml) and Rh IL-2 (10 ng/ml). β-Chemokine production by CD8⁺ T cells was analyzed in the culture supernatants after 3 days of PHA stimulation by ELISA. The indicated p-values were generated from t tests. For analyses of MIP-1α: *, P = 0.012, #, P = 0.004. For analyses of MIP-1β: *, P = 0.001, #, P = 0.008. The differences in RANTES values were not significant. Bars indicate the standard errors of the means.