Figure 3.

The effect of anti-β chemokine NAb on the CD8⁺ T-cell-mediated antiviral activity against the macrophage tropic HIV-1_BAL. The CD8⁺ T-cell culture supernatants obtained from 8 HIV⁺ subjects were assayed for antiviral activity in the presence of either a nonspecific control antibody (300 μg/ml) (■) or a mixture of NAb specific to RANTES (100 μg/ml), MIP-1α (100 μg/ml), and MIP-1β (100 μg/ml) (▨). The neutralization test was performed by incubating the antibody (R & D Systems) for 30 min at room temperature. Antibodies were added at the initiation of the culture and were replaced after 48 h on medium exchange. The effect on HIV replication was determined at day 8 by p24 ELISA.