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. 2000 Nov 21;97(25):13824–13829. doi: 10.1073/pnas.240303097

Figure 5.

Figure 5

Involvement of PI3K in Stat3-dependent and -independent migration of keratinocytes. (A) Costimulation with insulin and PMA promoted appreciable migration of either wild-type (+/+, black bars) or Stat3-disrupted (−/−, white bars) keratinocytes, either of which did not migrate by insulin or PMA alone. Migration of both types of cells was blocked by a PI3K inhibitor, wortmannin, in a dose-dependent manner. *, P < 0.05; **, P < 0.01 by Student's t test. (B) Wortmannin (50 nM) blocked 60%, 56% of the migration of wild-type cells promoted by EGF, HGF, respectively. **, P < 0.01 by Student's t test. (C) Western blot analyses revealed that stimulation not only of Stat3+/+ but also of Stat3−/− keratinocytes with HGF (50 ng/ml) or insulin (25 μg/ml) resulted in phosphorylation of Akt, a direct target of PI3K (16), indicating that PI3K activation in response to HGF or insulin was not affected by Stat3 ablation.