Figure 3.

L-selectin ligand activity of immunoprecipitated CD44. KG1a CD44 immunoprecipitates (Hermes-1 mAb; lanes 3 and 6) resolved on a reducing 9% SDS/PAGE gel, transferred to PVDF membrane, and immunostained with either Hermes-1 or HECA-452. Immunoblots included total cell lysate (lanes 1 and 4) and rat IgG isotype control immunoprecipitate (lanes 2 and 5) groups. L-selectin-dependent lymphocyte rolling was observed in the parallel-plate flow chamber (2.3 dynes/cm²) only over the 98-kDa protein (lymphocyte-binding data presented in parentheses as the mean number of lymphocytes rolling per field at ×200 magnification from more than five different fields).