Figure 5.
Effect of chlorate treatment on sulfation and L-selectin ligand activity of KG1a CD44. (A) SDS/PAGE (6%, reduced) autoradiogram of immunoprecipitated CD44 from non-chlorate- and chlorate-treated KG1a cells radiolabeled with [35S]SO4. (B) HECA-452 immunoblots of immunoprecipitated CD44 from chlorate-treated (+) and non-chlorate-treated (−) KG1a cells. L-selectin ligand activity of the 98-kDa band observed in the parallel-plate flow chamber (2.3 dynes/cm2) was equivalent in sulfated and nonsulfated (chlorate-treated) CD44 (binding data presented in parentheses as the mean number of lymphocytes at ×200 magnification per field; five fields counted).