Figure 1.

(a–c) Changes in secreted chemokine abundance after exposure of an epithelial cell line to B. pertussis for 24 h. Representative data from duplicate experiments are shown. Assays were performed for IL-8 (a), MCP-1 (b), and IL-6 (c). (d) Migration of neutrophils in response to the supernatants of B. pertussis-infected epithelial cells. In duplicate experiments, neutrophils migrated toward the supernatant with or without 10 μg/ml anti-IL-8 Ab. Migration index is the ratio of cells that migrated to a supernatant in comparison to the uninfected supernatant. Error bars represent the 95% confidence interval of the mean of three wells.