Table 1.
Epithelial cell transcriptional profile after exposure to B. pertussis
| Down-regulated
transcripts
|
Up-regulated transcripts
|
||||
|---|---|---|---|---|---|
| Ratio | Accession no. | GDB/other names | Ratio | Accession no. | GDB/other names |
| Chemokines/Cytokines | |||||
| +22.38 | X54489 | GRO1/SCYB1/MGSA | |||
| +16.29 | M28130 | IL8 | |||
| +9.11 | Y00787 | IL8 | |||
| +6.80 | X04500 | IL1B | |||
| +6.47 | N/A | MCP1/SCYA2 | |||
| M57731 | GRO2/SCYB2/MIP-2α | ||||
| X04602 | IL6/IFNB2 | ||||
| M23178 | SCYA3/MIP-1a | ||||
| X53800 | GRO3/SCYB3/MIP-2α | ||||
| Transcription factors/Zn-finger proteins | |||||
| −3.03 | X51435 | ZNF40/HIVEP1 | +7.39 | M59465 | TNFAIP3/A20 |
| D88613 | HGCM/GCMA | X65663 | SOX6 | ||
| U10324 | ILF3/NF90 | M82882 | ELF1/E74-like factor | ||
| N/A | MYBL1/A-MYB | U69127 | FUBP3/(FUSE)-binding protein 3 | ||
| X59244 | ZNF43/zinc-finger protein | U18259 | MHC2TA/CIITA-8 | ||
| X55544 | ATF1/TREB36 | ||||
| M29581 | ZNF8/zinc-finger protein 8 | ||||
| M96843 | ID2/inhibitor DNA binding | ||||
| U78722 | ZNF165/zinc-finger protein | ||||
| U28687 | ZNF157/zinc-finger protein | ||||
| N/A | BTF3 homologue | ||||
| Secreted molecules | |||||
| U19557 | SCCA2/protease Inhibitor | +4.59 | M57730 | EFNA1/LERK1/B61/Ephrin A1 | |
| U90546 | BTN3A2/butyrophilin | M31166 | PTX3/pentaxin-related/TSG-14 | ||
| X64877 | HFL3/H factor complement | X57579 | INHBA/inhibin, β A/activin | ||
| S75256 | LCN2/lipocalin 2 | X75308 | MMP13/collagenase 3 | ||
| M21642 | AT3/antithrombin III | M31551 | PAI2/plasminogen active inhibitor | ||
| ER/Golgi associated | |||||
| X97064 | SEC23A | L10333 | RTN1/reticulon 1 | ||
| X71661 | LMAN-1/ERGIC-53/lectin | U27326 | FUT3/fucosyltransferase 3 | ||
| L41390 | acetylglucosaminyltransfer | ||||
| U14550 | STHM/sialyltransferase | ||||
| Metabolic/synthetic | |||||
| −3.44 | AC002115 | COX6B/cytochrome c oxid. | D38524 | Cytosolic purine 5′-nucleotidase | |
| M61853 | CYP2C17/P450IIC17 | X78711 | GK/glycerol kinase deficiency | ||
| M60891 | UROD/decarboxylase | M61855 | CYP2C9/cytochrome P450, IIc | ||
| D49488 | TTPA/tocopherol α transfer | Z94753 | ATP7A/Cu++ ATPase | ||
| U75362 | USP13/ubiquitin protease | ||||
| S72370 | PC/pyruvate carboxylase | ||||
| X59834 | GLUL/glutamine synthase | ||||
| M84424 | CTSE/cathepsin E | ||||
| M64082 | FMO1/monooxygenase 1 | ||||
| D28791 | PIGA/phosphate inositol glycan | ||||
| D10040 | FACL1/fatty-acid-coA ligand | ||||
| Cell surface molecules/Receptors | |||||
| −4.76 | M32315 | TNFRSF1B/TNF receptor 2 | +4.53 | Y00285 | IGF2R/IGF II receptor |
| −3.85 | N/A | PSG11/glycoprotein | +4.30 | M24283 | ICAM1/CD54 |
| L36531 | ITGA8/integrin alpha 8 | +3.27 | U92971 | F2RL2/thrombin receptor-like | |
| M23197 | CD33 | S59184 | RYK/receptor tyrosine kinase | ||
| U64871 | GPR19/G protein-coupled | K02405 | HLA-DQB1/HLA-DC-3 β | ||
| U04811 | TRO/Trophinin | U11878 | CXCR2/IL8RB/IL-8 receptor | ||
| Y09392 | TNFRSF12/APO-3/TNFR | U65011 | PRAME/antigen of melanoma | ||
| X16983 | ITGA4/CD49D/integrin | ||||
| U61500 | TMEM1/EHOC-1 | ||||
| X77753 | M1S1/TROP-2/GA733-1 | ||||
| S67798 | SPAM1/sperm adhesion | ||||
| D31784 | CDH6/cadherin-6 | ||||
| S83249 | NG-TRA/hormone pump | ||||
| U80811 | EDG2/G-protein-coupled | ||||
| Cytoskeleton | |||||
| L07261 | ADD1/α adducin 1 | L20826 | PLS1/I-plastin | ||
| U34301 | Nonmuscle myosin heavy chain | ||||
| Intracellular signaling | |||||
| U48736 | PRP4h/ser-thr protein kinase | +3.47 | L42373 | PPP2R5A/protein phosphatase | |
| M38258 | RARG/retinoic acid receptor | +3.27 | M69043 | NFKBIA/IKBA/MAD-3 | |
| U18671 | STAT2 | U77129 | MAP4K5/KHS1/kinase | ||
| M35416 | RALB/v-ral homolog B | M80563 | S100A4/CAPL calcium binding | ||
| U40371 | PDE1C/phosphodiesterase | U37546 | API2/IAP homolog C/MIHC | ||
| U77845 | hTRIP/TRAF-interacting | ||||
| X78549 | PTK6/BRK | ||||
| U20158 | LCP2/SLP-76 | ||||
| U17327 | NOS1/nitric oxide synthase | ||||
| DNA replication/transcription | |||||
| U01833 | NUBP1/nucleotide binding | +3.31 | N/A | POLE/DNA polymerase, ɛ | |
| U47414 | CCNG2/cyclin G2 | X54993 | TBP/TFIID/TATA box binding | ||
| D26528 | DDX7/RNA helicase | X95152 | BRCA2/breast cancer 2 | ||
The experiment was repeated twice independently; data from one experiment are shown. Absolute difference ratios above or below the uninfected transcription are not reported when one of the two values was less than 2-fold the background noise level.