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. 2007 Jan 3;104(2):588–593. doi: 10.1073/pnas.0610115104

Fig. 6.

Fig. 6.

Prolonged IL-15 signaling in CD8 T cells by the membIL-15. Spleen cells from F5 TCR Tg mice were stimulated with the nominal peptide (NP68) in the presence of H-2b Ag-presenting cells for 2 days, followed by an 18-h cytokine-free culture to arrest them in a quiescent status. MACS-purified CD8 T cells were incubated with soluble rhIL-15 (A), rhIL-15 in the presence of IL-15Rα Tg DCs (B), or rhIL-15 with IL-15Rα−/− DCs (C). The cells were then permeabilized/fixed and analyzed for S6 phosphorylation by two-color flow cytometry. (A) Time kinetics of S6 phosphorylation to various doses of transpresented IL-15 (by membIL-15) in F5 CD8 T cells. (B) S6 phosphorylation to various doses of soluble IL-15 (soluble IL-15 with IL-15Rα−/− DCs). (C) S6 phosphorylation to various doses of soluble IL-15 (IL-15 without any DCs). (D) Kinetic comparison of the S6 phosphorylation pattern in CD8 T cells caused by soluble IL-15 and membIL-15.