Table 3.
Subcellular Fraction |
||
---|---|---|
Enzyme | Cytosol | Mitochondria |
G3PDH | 1.60 ± 0.25 | — |
Hexokinase | 0.04 ± 0.01 | — |
LDH | 1.30 ± 0.20 | — |
G6PDH | 0.03 ± 0.01 | — |
Malate dehydrogenase | 0.44 ± 0.08 | 0.22 ± 0.07 |
Aspartate aminotransaminase | 0.04 ± 0.01 | 0.03 ± 0.01 |
Glutamate dehydrogenase | ND | 0.07 ± 0.02 |
GS | ND | — |
Activities expressed as micromoles per minute per milligram protein; means ± SD. Each value is average of at least three experiments with triplicate culture dishes per experiment. ND, not detected.