Figure 2  .

Mature dendritic cells (DC) were left uninfected, infected with Adv0, or infected with AdvIκBα at a multiplicity of infection of 300. DC were then plated in graded doses for 10⁵ purified, allogeneic T cells in triplicate in a 96 well, round bottom microtitre plate on day 1 after adenovirus infection. Proliferation was determined on day six with either a 5-bromo-2'-deoxyuridine (BrdU) labelling and detection kit III (Boehringer Mannheim, East Sussex, UK) (A) or by a [³H]thymidine ([³H]TdR) uptake assay (B). Each point represents the mean (SEM) of either six (A) or three (B) separate experiments.