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Confirmation of lp25 and lp28–1 plasmid content in 19 B. burgdorferi B31 clones by Southern blot analysis. PCR-amplified probes from two different regions of each plasmid (marked 1° and 2°) were labeled and hybridized to Southern blots of agarose gel-electrophoresed plasmid preparations from each of the 19 clones. The positions of the hybridizing bands corresponded precisely with lp25 and lp28–1, respectively (data not shown).
