Figure 3.

Immunohistochemical staining for the mutant huntingtin protein (EM-48) (a and b), ubiquitin (nuclear inclusion marker) (c), and HLA-DR (microglia and macrophages marker) (d) in the transplant and the host tissue. EM-48 staining of mutant human huntingtin protein, specifically labeling nuclear inclusions, is abundant in the host tissue (b) but not in the transplant (a). Inclusions in b are illustrated at higher magnification in the Inset. (c) Low-power photomicrograph of ubiquitin staining in the host (h) and absence of staining in the transplant (tp) (interface of the transplant and the host indicated by the dotted line). The transplant (left) is clearly devoid of ubiquitin expression. (d) Low-power photomicrographs of HLA-DR staining (interface of the transplant and the host indicated by a dotted line). The transplant contains few HLA-DR positive cells (tp) compared with the host tissue (h), which contains numerous HLA-DR immunoreactive cells. (Scale bars: a and b, 50 μm; Inset, 25 μm; c, 500 μm; d, 100 μm.)