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. 2006 Dec 22;7:48. doi: 10.1186/1471-2199-7-48

Table 1.

Summary of proteins identified by peptide mass fingerprint and peptide sequencing

Band (molecular weight) Proteins, abbreviation, and SwissProt accession number Number of sequenced peptides
1 (110 kDa) - Poly (ADP-ribose) polymerase 1 (PARP-1; P09874) 3 (m)
2 (75 kDa) - ATP-dependent helicase II (Ku80; P13010) 1 (m)
3 (65 kDa) - ATP-dependent helicase II (Ku70; P12956) 2 (m)
4 (60 kDa) - PARP-1
- Ku70
- Keratin
2 (LC)
2 (LC)
5 (50 kDa) - PARP-1
- Keratin
6 (LC)
6 (35 kDa) - DNA-(apurinic or apyrimidine site) lyase (APEX1; P27695)
- Glyceraldehyde-3-phosphate hydrogenase (GAPDH; P04406)
9 (LC)
3 (LC)
7 (25 kDa) - PARP-1 9 (LC)

Labels "m" refer to verification by manual ESI-MS/MS and "LC" to HPLC-MS/MS, respectively.