Table 1.
Himar 1 gene interruptions that attenuate survival of B. melitensis 16 M in J774.A1 macrophages assorted according to COG‡
| Functional Class | B. melitensis (BME) locus* | Genes Identified (#) | Fraction Identified (%) | Novel Genes (#) | Target Specificity† |
| Intracellular trafficking, secretion | II0025, II0026, II0027, II0028, II0029, II0029/30 II0030, II0032, II0033, II0034, II0035 | 11 | 12% | 3 | 12.8 |
| Nucleotide transport and metabolism | I0233, I0295, I0296, I1123, I1124, I1127, I1204, I1240, I1488, I1519 | 10 | 11% | 5 | 3.5 |
| Signal transduction | I0066, I1296, I1327, I1807, II0011 | 5 | 5% | 3 | 2.4 |
| Transcription | I0304/5, I0371, I0508, I0513, I0513/4, I0731/2, I0808, I1178, I1297/8, I1364, I1647/8, II1116, II1116/7 | 13 | 14% | 11 | 2.1 |
| Cell wall/membrane/envelope biogenesis | I0498/9, I0997, I1302, I1326, I1393, I1413, I1414, I1415, I1416, I1426, I1427, I1886, II0380, II0472, II0899 | 15 | 16% | 7 | 1.9 |
| Energy production and conversion | I0972, I1749, II0428, II0429, II0759, II0760, II0761, II0762 | 8 | 8% | 2 | 1.2 |
| Amino acid transport and metabolism | I0025, II0039, II0040, II0285, | 4 | 4% | 1 | 1.0 |
| Replication, recombination and repair | I0040, I0334, I1307, I2023, II0260 | 5 | 5% | 4 | 0.5 |
| Translation, ribosome structure and biogenesis | I0983, I1057, I1775/6 | 3 | 3% | 3 | 0.4 |
| Function unknown | I0186, I0193, I0490, I0540, I0603, I0732, II0045/6 | 7 | 7% | 7 | 0.4 |
| Posttranslational modification, protein turnover, chaperones | I0816, I1804,II0932 | 3 | 3% | 2 | 0.4 |
| Defense mechanisms | I0926 | 1 | 1% | 1 | 0.3 |
| General function prediction | I1143, I1282, I1487, I1499, I1867, I1894 | 5 | 5% | 5 | 0.2 |
| Carbohydrate transport and metabolism | I2031, II1095 | 2 | 2% | 1 | 0.2 |
| Lipid transport and metabolism | I1553 | 1 | 1% | 0.1 | |
| Coenzyme transport and metabolism | I0657 | 1 | 1% | 1 | 0.1 |
‡ Genes assigned to COGs that are not listed were not identified.
* Numbers in bold reflect novel identification (n = 57). Those that are not bold have been identified previously using Tn5 by us and others [46]
/ Indicates intergenic insertion between two genes.
† ts or target specificity represents the ratio of insertions per COG divided by the fraction of the genome represented by that COG.