Figure 1.

Caspase-1 cleavage appears early in the lifespan of G37R- and G85R-transgenic mice. Immunoblots of spinal cord extracts from G37R (a) and G85R (b) mice probed with a polyclonal antibody against both the inactive (p45) pro-enzyme and the active (p20) caspase-1. In mice over-expressing the wt human SOD1 (hSOD1) protein, the antibody only recognized the p45 inactive and the p30 intermediate form. (c) Caspase-1 cleavage in G37R mice detected with the polyclonal antibody used for the cell culture experiments. wt indicates mice overexpressing wt hSOD1.