Figure 3.

Labeling of consecutive coronal sections through the anterior preoptic area, showing the specificity of the immunocytochemical reactions. (A and B) Specificity control of the 3β-HSD immunostaining. Adjacent sections were incubated with the antibodies against 3β-HSD (A) or with the antibodies preabsorbed with purified human type I 3β-HSD (10⁻⁶ M) (B). (C and D) Specificity control of the α₃ subunit immunostaining. Adjacent sections were incubated with the antibodies against the α₃ subunit (C) or with the antibodies preabsorbed with the purified peptide hapten (10⁻⁶ M) (D). (Bars = 10 μm.)