Effects of GABA (10−6 M) in the absence or presence of the
GABAA antagonists SR95531 (10−5 M) and
bicuculline (10−5 M) on the conversion of
[3H]pregnenolone into 17-hydroxypregnenolone and
dihydrotestosterone (17OH-Δ5P/5α-DHT;
A), dehydroepiandrosterone (DHEA; B),
17-hydroxyprogesterone (17OH-P; C), and progesterone (P;
D) by hypothalamic slices. The values were obtained from
experiments similar to those presented in Fig. 4. Results are expressed
as percentages of the amount of each steroid formed in the absence of
drugs. Each value is the mean (±SEM) of four independent experiments.
*, P < 0.05; **,
P < 0.01; ***,
P < 0.001 by one-way ANOVA followed by a post hoc
Bonferroni's test; NS, not statistically different.