Figure 1.

Example of the Raman modelling procedure for quantification of proteins, calcification, and lipids, as described by Brennan and colleagues.⁴ (A) Raman spectrum after correction for background signal. This spectrum was obtained in 60 seconds from an atherosclerotic specimen using the fibreoptic catheter system. (B) Raman model spectra. From top to bottom: β carotene, calcification, adventitial fat, esterified cholesterol, cholesterol, delipidised atherosclerotic artery (protein 2), delipidised normal artery (protein 1). (C) Raman spectrum (black) and model fit (grey). The fit parameters are shown in the inset.