Zymography of matrix metalloproteinases (MMP) in the LETO group (lanes 1 to 4), in the OLETF group (lanes 5 to 8), and in the OLETF+ARB group (lanes 9 to 14). The latent type (72 kDa) and active type (62 kDa) of MMP-2 tended to decrease in the OLETF group, an effect that was significantly reversed by angiotensin II receptor blockade. The bar graphs show active MMP-2 and total MMP-2, defined as the sum of latent and active type, which were quantified with the NIH image and expressed as μU/μg protein. ARB, angiotensin II receptor blockade.