Figure 1.

Cell lysates of GT1 lines were loaded onto 12% polyacrylamide gel, transferred onto poly(vinylidene difluoride) membrane, and PrP^C detected with antibody P45–66 raised against the amino terminus of the protein (A) or with a mixture of mAb (SAF 60, SAF 69, and SAF 70) raised against the carboxy terminus of the protein (C). PrP^Sc was detected with the same mix of mAbs but after proteinase K digestion (B). Molecular mass markers are indicated on the left in kDa. In D, DNA fragmentation was demonstrated in infected cells by the DNA laddering technique.