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. 2003 Oct;89(10):1195–1199. doi: 10.1136/heart.89.10.1195

Table 2.

Polymerase chain reaction primers and conditions

Polymorphism Primer sequence and concentration Annealing temperature (°C) MgCl2 (mM)
ACE I/D ACE1 (D allele specific): 5′-CATCCTTTCTCCCATTTCTC, 800 nM 50 1.5
ACE2 (I allele specific): 5′-TGGGATTACAGGCGTGATACAG, 800 nM
ACE3: 5′-ATTTCAGAGCTGGAATAAAATT, 240 nM
AGT T174M Forward inner primer (T allele specific): 5′-CTGGGGCTGTGAACACGCCCACCAACA, 100 nM 55 2
Reverse inner primer (C allele specific): 5′-TGATAGCCAGGCCCAGCTGCTGCTGTCAAC, 100 nM
Forward outer primer : 5′-TCTCCCCAACGGCTGTCTTTGGCACCCT, 10 nM
Reverse outer primer: 5′-CACTGGCTCCCATCAGGGAGCAGCCAGT, 10 nM
AGTR1 +1166 A>C Forward inner primer (A allele specific): 5′-TCTGCAGCACTTCACTACCAAATGAACA, 1000 nM 58 3.5
Reverse inner primer (C allele specific): 5′-TCTCCTTCAATTCTGAAAAGTAGCTGAG, 1000 nM
Forward outer primer: 5′-GCCAAATCCCACTCAAACCTTTCAACAA, 100 nM
Reverse outer primer: 5′-AAGCAGGCTAGGGAGATTGCATTTCTGT, 100 nM
AGTR2 +1291G>T Forward inner primer (T allele specific): 5′-TTGTTTTTCTGATATGTTTGTACAAGACTT, 600 nM 54 3.5
Reverse inner primer (G allele specific): 5′-TCTAGGTTGTAAATATGTCTCACYAATAAC, 600 nM
Outer forward primer: 5′-AATAAAATTTCCCCTAATCTTTTCTGAA, 200 nM
Outer reverse primer: 5′-GGAAATCCTGATAAAAATGGATATAAAA, 200 nM
AGTR2 +1297G>A Forward inner primer (G allele specific): 5′-TCTGATATGTTTGTACAAGATTKTCAGTG, 600 nM 56 3.5
Reverse inner primer (A allele specific): 5′-GTTACTTCTAGGTTGTAAATATGTCTCCCT, 600 nM
Outer forward primer: 5′-AATAAAATTTCCCCTAATCTTTTCTGAA, 200 nM
Outer reserve primer: 5′-GGAAATCCTGATAAAAATGGATATAAAA, 200 nM

ACE, angiotensin converting enzyme; AGTR1, angiotensin II type 1 receptor; AGTR2, angiotensin II type 2 receptor; D, deletion; I, insertion.