Figure 2.

The carboxyl-terminal TMR of syntaxin 1A is essential for N-type Ca²⁺ channel modulation. The mutant constructs shown (Left) are described in Results and Materials and Methods. Striped areas denote various TMRs taken from syntaxin 2, syntaxin 2′, syntaxin 3, and the carboxyl-terminal region of syntaxin 2′′. Crosshatched area denotes a scrambled version of the TMR of s1A. Bar graph displays mean ± SEM values of the I₈₀/I₁₂₀ ratio for each construct (number of oocytes shown in parentheses).