Figure 1.

Intense cyclo-oxygenase-2 (COX-2) myocardial staining. Intense cytoplasmic staining is shown in several cardiomyocytes at the site of infarction, using a primary anti-COX-2 antibody (goat polyclonal sc-1745, Santa Cruz Biotechnology, California, USA, at a 1:100 dilution, according to the results of titration experiments for optimal dilutions), and with a secondary reaction using the streptavidin–biotin system (Dako, Carpintera, California, USA), with diaminobenzidine as the final chromogen.