Figure 2.

 (A) UFH inhibited thrombin but not adrenaline induced platelet calcium fluxes. (B) UFH did not affect thrombin receptor activating peptide (TRAP)-6 induced calcium fluxes. (C) Abciximab, but not UFH or LMWH, inhibited TRAP-6 induced platelet aggregation. Washed platelets (4 × 10⁷−8 × 10⁷) were preincubated with UFH, LMWH, or abciximab for five minutes before addition of agonist. Aggregation was carried out for 300 seconds. Traces are representative of 10 similar experiments. Panels (A) and (B) show representative fluorimetry traces indicating calcium influx on an arbitrary scale based on Fura-2 loaded platelets (4 × 10⁷−8 × 10⁷). Calcium flux was read over 300 seconds. Traces are representative of five similar experiments.