Figure 1.

Interactions between CREB2, ATFx, and GABA_B receptor subunits, as determined by β-galactosidase activity in the YTH system. CREB2 and ATFx GAL4_AD fusion protein and GABA_B receptor C termini GAL4_BD fusion proteins were coexpressed in the YTH system, and β-galactosidase activity was determined. The regions of the GABA_B-R1 expressed were amino acids 854–961 (GABA_B-R1a) and amino acids 877–928 for the coiled-coil (CC) domain. The amino acids expressed from the GABA_B-R2 C terminus were 744–941 and 779–847 for the coiled-coil domain. The intensity of the resulting β-galactosidase activity is indicated by the number of + signs. The relative positions of the bZIP domain and the putative MAPK phosphorylation sites are shown as hatched boxes and star symbols, respectively.