Figure 4.
GABAB receptor activation alters CREB2 localization in CHO-GABAB cells. (a) CHO-ORL1 cells show bright nuclear localization of eGFP-CREB2. (b) CHO-GABAB cells show diffuse and punctate cytoplasmic fluorescence for eGFP-CREB2 as well as bright nuclear fluorescence. Treatment with 100 μM baclofen significantly decreases cytoplasmic eGFP-CREB2. (c) GABAB antagonist CGP54626 (1 μM) blocks the effects of baclofen (100 μM). (d) Immunoblots of cytoplasmic extracts prepared from CHO-GABAB cells, transfected with CREB2, show decreased levels of CREB2 in the cytoplasm after baclofen treatment (100 μM), whereas CGP54626 (1 μM) partially blocked the decrease in CREB2 levels. Cytoplasmic levels of GABAB-R1 remained constant.