Figure 5.

Baclofen increases CREB2 levels in the nuclei in primary rat cortical cultures. Treatment of cortical cultures with baclofen (20 μM) gave a 3-fold increase in CREB2 immunoreactivity in nuclear extracts in contrast to cytoplasmic extracts, as determined by Western blotting. The nuclear localization of CREB2 was blocked by the GABA_B receptor antagonist CGP54626 (1 μM). Sc-35 localized entirely to the nuclear extract and showed no alteration in levels with baclofen treatment. Means of four independent experiments reveal a 3.3 ± 1.3 (SD) increase in nuclear CREB-IR after baclofen treatment.