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. 2000 Nov 28;97(25):13973–13978. doi: 10.1073/pnas.250483897

Figure 3.

Figure 3

(A) Effects of various concentrations of somatostatin-28 and analogs on forskolin-stimulated cAMP accumulation in cells expressing sst3. Concentration–response curves were obtained with increasing concentrations of somatostatin-28 (●), ODT-8 (■) or 9 (▴), and of somatostatin-28 in the presence of 10−5 M 9 (▾),10−5 M 10 (○), or 10−5 M ODT-8 (♦). Data are expressed as a percentage of the 10 μM forskolin response. The somatostatin analogs 9 and 10 antagonize the concentration-dependent inhibition of cAMP accumulation by somatostatin-28 in sst3-expressing cells. (B) Effects of various concentrations of somatostatin analogs in the presence of a fixed concentration of somatostatin-28 on forskolin-stimulated cAMP accumulation in cells expressing sst3. Concentration–response curves were obtained with increasing concentrations of 7 (●), 10 (■), and ODT-8 (▴) in the presence of 5 × 10−8 M somatostatin-28. Data are expressed as a percentage of the 10 μM forskolin response. The somatostatin analogs 7 and 10 concentration-dependently reverse the inhibition of cAMP accumulation by somatostatin-28 in sst3-expressing cells. (C) Effects of various concentrations of 10 on somatostatin-28-stimulated IPx accumulation in cells expressing sst3. The concentration–response curve was obtained with increasing concentrations of 10 in the presence of 10−5 M somatostatin-28. Data are expressed as a percentage of the 10 μM somatostatin-28 response. 10 antagonizes concentration-dependently the stimulation of IPx accumulation by somatostatin-28 in sst3-expressing cells.