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All three AtXRNs function as exoribonucleases, which are blocked by poly(G) tracts when expressed in yeast. The AtXRNs were expressed in the xrn1Δ strain, and the accumulation of the poly(G) reporter mRNAs PGK1 (Top) and MFA2 (Bottom) was analyzed by Northern blot. The structure of the poly(G) reporters is shown at Right, and the AtXRN expressed in the xrnΔ strain is shown above.
