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Double immunohistochemical staining of 5 μm sections of a coronary endarterectomy specimen showing co-localisation of matrix metalloproteinase-9 (MMP-9) and C pneumoniae in the arterial wall. Sequential sections stained with FITC labelled antibodies to (A) C pneumoniae (RR402) and (C) MMP-9. (B, D) The same sections stained with 7-amino-4-methylcoumarin-3-acetate (AMCA) labelled CD68 antibody. Arrows indicate positively stained cells. (Original magnification ×400).
