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. 2005 May;91(5):664–669. doi: 10.1136/hrt.2003.031898

Figure 2.

Figure 2

 E2 down regulates Ang II induced endothelin-1 (ET-1) gene expression in cardiac fibroblasts. The results are shown as the mean (SEM) (n  =  6). *p < 0.05 v C; †p < 0.05 v Ang II alone. (A) Downregulation of Ang II induced ET-1 mRNA by E2. Cells were preincubated with α-E (100 nmol/l), E2 (100 nmol/l), or the combination of E2 plus ICI (1 µmol/l) and then stimulated with Ang II (100 nmol/l) for 30 minutes or not. Total RNA was extracted and northern hybridisation was performed with 32P labelled ET-1 as a probe. 18S RNA was used to normalise the RNA applied in each lane. Data are presented as percentage changes of experimental groups compared with untreated C. (B) E2 inhibits Ang II induced ET-1 promoter activity. Cells were transfected with chimeric chloramphenicol acetyltransferase (CAT) fusion genes and preincubated with α-E (100 nmol/l), E2 (100 nmol/l), or the combination of E2 plus ICI (1 µmol/l) and then stimulated with Ang II (100 nmol/l) for 24 hours or not. Cells were harvested and CAT activities were measured. CAT activities after normalising to activities of β-galactosidase are shown as activity relative to C. CAT2 and CAT3 are positive and negative controls, respectively.