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. Author manuscript; available in PMC: 2007 Jan 12.
Published in final edited form as: J Biol Chem. 2006 Sep 12;281(45):34537–34548. doi: 10.1074/jbc.M606654200

FIGURE 3. Phosphorylation of Pah1p on Ser/Thr-Pro sites is required for efficient transcriptional dere-pression of phospholipid biosynthetic genes.

FIGURE 3

A, pah1Δ cells expressing the wild-type PAH1 or the PAH1-7P allele from centromeric vectors were grown in synthetic medium supplemented with 10 Δ g/ml inositol to early logarithmic phase. Cells were then transferred into medium lacking inositol, and the mRNAs of INO1 (left panel), OPI3 (right panel), and RTG2 (control) were quantified at the indicated time points by real time RT-PCR. Values are given as INO1:RTG2 and OPI3:RTG2 ratios. Values and errors were calculated from two independent experiments. WT, wild type. B, serial dilutions of wild-type cells transformed with the indicated plasmids were spotted on synthetic plates lacking inositol and supplemented with glucose (left) or galactose (right). Cells were grown for the indicated times at 30 °C.