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. Author manuscript; available in PMC: 2007 Jan 12.
Published in final edited form as: J Biol Chem. 2006 Sep 12;281(45):34537–34548. doi: 10.1074/jbc.M606654200

FIGURE 6. Cooperative effects of pah1Δ and opi1Δ on the derepression of phospholipid biosynthetic genes.

FIGURE 6

A, the mRNA levels of INO1 were analyzed in the wild-type and isogenic pah1Δ, opi1Δ, and pah1Δ opi1Δ strains grown in YEPD medium by quantitative real time RT-PCR. Amplification of each sample was performed in duplicate and normalized to ACT1. Values and errors were calculated from three independent experiments performed on different days. Values are expressed as –fold derepression over the values measured in the wild-type strain. B, the OPI3 transcripts were quantified as in A. C, serial dilutions of the wild-type and isogenic pah1Δ, opi1Δ and pah1Δ opi1Δ strains were spotted on YEPD plates and grown at 30 °C for the indicated times.