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. Author manuscript; available in PMC: 2007 Jan 12.
Published in final edited form as: J Biol Chem. 2006 Sep 12;281(45):34537–34548. doi: 10.1074/jbc.M606654200

FIGURE 7. Phospholipid overproduction is not sufficient for nuclear membrane growth.

FIGURE 7

SEC63-GFP and RFP-PUS1 expressed from centromeric plasmids were used to visualize nuclear/ER membrane and nuclear morphology respectively in wild-type cells (A), pah1Δ cells (B), opi1Δ cells (C), wild-type cells transformed with high copy vectors expressing INO2 and INO4 under the control of the inducible MET25 promoter (D) and wild-type cells transformed with a centromeric vector expressing HMG1 under the control of the inducible GAL1/10 promoter (E). Cells in D were grown in medium lacking methionine and in E in medium supplemented with 2% galactose to induce INO2-INO4 and HMG1, respectively. In all cases cells in early logarithmic phase were visualized by confocal microscopy. Bars, 5 μm.