TABLE 2.
Plasmids used in this study
| Plasmid | Description | Reference/source |
|---|---|---|
| YCplac111-PAH1 | PAH1 into CEN/LEU2 vector | 17 |
| YCplac111-PAH1-PtA | PAH1-PtA into CEN/LEU2 vector | 17 |
| YCplac111-PAH1-7P-PtA | PAH1-PtA with the following mutations (7P): S110A/S114A/S168A/S602A/T723A/S744A/S748A cloned into CEN/LEU2 vector | This study |
| YCplac111-PAH1-NCK-7P-PtA | PAH1-PtA with the following mutations (NCK-7P): S773A/S774A/S800A/S805A/S810A/S814A/S818A cloned into CEN/LEU2 vector | This study |
| YCplac111-PAH1-7P | PAH1-7P cloned into CEN/LEU2 vector | This study |
| YEplac181-GAL1/10-PAH1 | PAH1 under the control of GAL1/10 promoter, cloned into 2-μ/LEU2 vector | This study |
| YEplac181-GAL1/10-PAH1-7P | PAH1-7P under the control of GAL1/10 promoter, cloned into 2-μ/LEU2 vector | This study |
| YEplac181-GAL1/10-PAH1-NCK-7P | PAH1-NCK-7P under the control of GAL1/10 promoter, cloned into 2-μ/LEU2 vector | This study |
| YEplac181-GAL1/10-OPI1 | OPI1 under the control of GAL1/10 promoter, cloned into 2-μ/LEU2 vector | This study |
| YEplac181-GAL1/10-PAH1-PtA | PAH1-PtA under the control of GAL1/10 promoter, cloned into 2-μ/LEU2 vector | This study |
| YEplac181-GAL1/10-PAH1-7P-PtA | PAH1-7P-PtA under the control of GAL1/10 promoter, cloned into 2-μ/LEU2 vector | This study |
| YCplac111-SEC63-GFP | SEC63-GFP into CEN/LEU2 vector | This study |
| YCplac33-RFP-PUS1 | RFP-PUS1 under the control of the NOP1 promoter, cloned into CEN/URA3 vector | This study |
| PJS470 | INO2 under the control of the MET25 promoter into a 2-μ/TRP1 vector | 30 |
| PJS471 | INO4 under the control of the MET25 promoter into a 2-μ/HIS3 vector | 30 |
| YCplac111-GAL1/10-HMG1 | HMG1 under the control of the GAL1/10 promoter cloned into a CEN/LEU2 vector | This study |