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. Author manuscript; available in PMC: 2007 Jan 12.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2005 Jun;46(6):1882–1890. doi: 10.1167/iovs.04-1286

Figure 3.

Figure 3

Immunoblot analysis of rpgrip1b in murine tissues. Antibodies against the seven C-terminal residues of rpgrip1b (A, B) and the coiled-coil domain of RPGRIP1/rpgrip1b (C) detected a major protein with the predicated and apparent molecular mass of 63 kDa (A) and with the expression restricted to the murine retina (B). The 63-kDa protein was not immunoreactive to the rpgrip1b Ab preadsorbed with the cognate peptide epitope and an antibody (Ab38) against the C-terminal RID domain of RPGRIP1 (data not shown). The Ab22 detected also a testis-restricted and very abundant rpgrip1 isoform (rpgrip1T110) with an apparent molecular mass of 110 kDa (D), which was also immunoreactive to an antibody (Ab38) against the C-terminal RID domain of RPGRIP1 (E).