Table 1.
Complementation analysis
| % mutant pollen | KmR:KmS T2 |
pptR:pptS F1 |
% pptR F1 |
|
| pMSP1-TIO-3 | 28.7 | 48:12 | 30:61 | 32.96 |
| pMSP1-TIO-9 | 14.5 | 103:5 | 284:294 | 49.13 |
| pMSP1-TIO-10 | 11.9 | 91:10 | 193:237 | 44.88 |
| pMSP1-TIO-18 | 27.9 | 56:18 | 43:90 | 32.33 |
| pMSP2-TIO-1 | 40.3 | 323:67 | 41:145 | 22.04 |
| pMSP2-TIO-2 | 43.3 | 91:15 | 29:156 | 15.68 |
| pMSP2-TIO-5 | 29.2 | 88:3 | 29:60 | 32.58 |
| pMSP2-TIO-8 | 46.6 | 85:31 | 11:131 | 7.75 |
| +/tio-3 | 50.6 | - | 0:215 | 0 |
Four lines harbouring each construct were compared to the heterozygous parent plant, tio-3. The % of mutant pollen was scored after DAPI staining and the T2 segregation of the complementing T-DNA (MSP-TIO) was analysed on kanamycin media. The extent of male transmission (% pptR) of tio-3 was determined on ppt media in F1 progenies from test-crosses.