Table 2.
Primer | Primer sequence |
cDNA construction | |
3'-RACE | 5'-AAGCAGTGGTAACAACGCAGAGTAC(T)30VN-3' |
RT-PCR reverse primer | |
NESTED | 5'-AAGCAGTGGTAACAACGCAGAGT-3' |
RT-PCR forward primers | |
At5g40040 | 5'-CTTATCGCTGTTGGACGAGAGAAGA-3' |
At5g59040 | 5'-TCTGTCTCGCCGTCATTTTTGTTAT-3' |
At5g46795 | 5'-GGCTTTGGAGACCAGACTTTTTCAG-3' |
At4g26440 | 5'-TGGAGAGGTAGAAGAGTCCGAATCA-3' |
At2g03170 | 5'-AGAAACACGTCGTTGACGAAGAAAG-3' |
At3g14450 | 5'-GGCCAAGGAGTTTTTCCCTTCTTA-3' |
At1g53650 | 5'-CTCGATCATTGAGCTCAGAAGCTGT-3'. |
Construction of promoter::GUS reporters | |
MSP1-F | 5'-AAAAAGCAGGCTTGTCAGTTAGCATGAAAAATTGTATGTTAG-3' |
MSP1-R | 5'-AGAAAGCTGGGTTTGTTGTGTATACTTGTGTGTGTGTATTTA-3' |
MSP2-F | 5'-AAAAAGCAGGCTATGTCCTACGATCAGAAGGAGGAG-3' |
MSP2-R | 5'-AGAAAGCTGGGTAACATGTGATATTATTTTTTTGGTTTATATAGTGG-3' |
MSP3-F | 5'-AAAAAGCAGGCTTTGTGATATAATAGGTATATATGGTAGAAC-3' |
MSP3-R | 5'-AGAAAGCTGGGTTGCAAACCCAAGTTTCAGCTTTAAC-3' |
Primers used for cDNA synthesis, RT-PCR analyses and construction of promoter::GUS reporters. Where appropriate, appended adapters complementary to AttB1 and AttB2 primers are underlined.