Figure 1. IL-4Rα Expression Is Impaired on Smooth Muscle Cells in SM-MHC^CreIL-4Rα^−/lox Mice.

(A) Genomic integrity of the SM-MHC^CreIL-4Rα^−/lox hemizygous mice was established by PCR.

(B) Smooth muscle cells were identified by intracellular α-actin (i) staining versus isotype control (ii). IL-4Rα surface expression was analyzed on gated α-actin–positive cells (iii).

(C) cDNA levels in trachea and small intestine of IL-4Rα^−/lox (black bars) and SM-MHC^CreIL-4Rα^−/lox (hatched bars). Data are derived from pooled tissue samples from three mice and are representative of two experiments.

(D) IL-4Rα expression on lymphocyte subpopulations of T cells and B cells is unaffected in SM-MHC^CreIL-4Rα^−/lox mice.