Figure 3. CD4⁺ Lymphocytes Are Essential for N. brasiliensis Clearance.

MLNs were removed from infected mice on days 4, 7, and 10 PI. CD4⁺ cells were isolated and stimulated with CD3 for 72 h. Supernatants were then analyzed for cytokine production by ELISA as described in Materials and Methods. *Significant difference (p < 0.05) from IL-4Rα^−/lox mice. Data are representative of four repeated experiments.