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. 2007 Jan 16;5(2):e20. doi: 10.1371/journal.pbio.0050020

Figure 1. Analysis of the Unwinding and Strand Annealing Activities of RECQ1.

Figure 1

(A) Kinetics of unwinding performed using 5 nM RECQ1.

(B) RECQ1 unwinding at varying enzyme concentrations (15-min reaction).

(C) Kinetics of strand annealing in the presence of 20 nM RECQ1 and in the absence of enzyme (open diamond [◊]).

(D) Analysis of RECQ1 strand annealing activity as a function of protein concentration (15-min reaction). Data points were the mean of three independent experiments with the standard deviation indicated by error bars. The concentration of ATP in all the experiments varied from 0 to 5 mM (filled circle [•] = 0 mM, open inverted triangle [▿] = 0.5 mM, filled triangle [▴] = 1 mM, open circle [○] = 2 mM, and filled square [▪] = 5 mM).