Table 1.
Basic features of the three polymorphic markers on Coja region
| Coja haplotype 1 (Acc. Num. AB078884) | |||||||
| marker name | Nuc. position** | Gene position | length (bp) | range (bp) | polymorphisms | primer name | primer sequence (5' to 3') |
| PM1 | 89043 – 89887 | Tapasin (ex.4 – int.4) | 845 | 718–1095 | (ATGAT)n (TTCCTATGGGGGCTGTAGGGTGGATGGGACTGGGTGGTA)n |
TAPBPL-F | CAGGTCCTGCTGGCCTATGA |
| TAPBPL-R | TGGTGTGATGCCAACCCAT | ||||||
| PM2 | 92768 – 93787 | Coja-DBB1 (Promoter – ex.3) | 1002 | 989–1002 | 126 SNPs, 13 indels | CojaDBBpromoterF6 | CCCTGGGGACACCATTTGTCAT |
| C2BNO5 | GCGCCAGGAAGACGAGCCCCAGCAC | ||||||
| PM3* | 118204 – 118536 | DMB2 (int.1 – int.2) | 333 | 333 | G/A, G/C | DMB2-F | GGAGTGCATCCCCATTGCT |
| DMB2-R | GCTCACTCTTGCGCAGTGC | ||||||
* PM3 contains two SNP sites.
** positions on Coja haplotype 1 genomic sequence (accession number; AB078884).