Figure 7.

Addition of agonist leads to the redistribution of β-arrestin 1-GFP to early endosomal structures in the cytosol. HeLa cells were transiently transfected with human FLAG-tagged M₂ mAChR and β-arrestin 1-GFP and treated with 1 mM carbachol for 30 minutes. Cells were processed for confocal microscopy. β-arrestin 1-GFP complexes localized to the early endosome as shown by colocalization with markers of that compartment (EEA-1 and TfnR). Arrows indicate signficant overlap between TfnR or EEA-1 with β-arrestin 1-GFP. Confocal images are representative of three independent experiments.